Scrape loading dye transfer assay

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WebSep 18, 2014 · Scrape-loading/dye transfer assay for gap junctional intercellular communication Solutions needed: HBSS + 1% BSA (50 ml; 0.5 g BSA/HBSS) PBS + 1% … WebJul 1, 2008 · The scrape loading and dye transfer (SL/DT) method is a functional assay widely used to evaluate the level of the intercellular communication via gap junction. Initially, the evaluation of the degree of communication was done by counting the rows of fluorescent cells from the scrape.

[PDF] Applicability of Scrape Loading-Dye Transfer Assay for Non ...

WebAug 1, 2024 · The SL-DT assay can serve a broad range of in vitro pharmacological and toxicological needs, and can be potentially adapted for high throughput set-up systems … WebJul 1, 2008 · The scrape loading and dye transfer (SL/DT) method is a functional assay widely used to evaluate the level of the intercellular communication via gap junction. … diy child proof cabinet locks

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WebJul 1, 2008 · The scrape loading and dye transfer (SL/DT) method is a functional assay widely used to evaluate the level of the intercellular communication via gap junction. Initially, the evaluation of the degree of communication was done by counting the rows of fluorescent cells from the scrape. WebAug 20, 2024 · Among various techniques developed for in vitro assessment of GJIC, the SL-DT (i.e., scrape loading-dye transfer) assay has probably been most frequently used in multiple studies of toxicant or carcinogen effects on GJIC. This in vitro assay is applicable to various cell types and cell lines. WebMar 8, 2024 · In scrape-loaded astrocytes, the dye traveled through functional gap junctions into adjacent gap junction–coupled astrocytes that avoided the scrape-loading. Astrocytes were pretreated with MHC1 (66.7 nM) or a known gap junction blocker, carbenoxolone (CBX) (100 μM), for either 3 or 24 hours at 37°C prior to the gap junction coupling assay. diy chick starter feed

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WebOne of these assays, the scrape-loading dye transfer (SLIDT) technique, is based on monitoring the transfer of the fluorescent dye Lucifer yellow from one cell into adjacent cells via functional gap junctions. The objective of our study was to evaluate and compare various approaches for quantifying results obtained with the SL/DT technique. WebJun 1, 2000 · The scrape-loading dye transfer assay allows simultaneous monitoring of dye coupling in a large population of cells; its suitability for the assessment of gap junction-mediated intercellular... diy child play kitchenWebThe scrape loading/dye transfer (SL/DT) technique is a simple functional assay for the simultaneous assessment of gap junctional intercellular communication (GJIC) in a large … craigmar weddings

"WebMay 21, 2016 · Scrape-load dye-transfer assay was performed as previously described using 5 minutes room temperature incubation time post-scrape. 57 Cells were washed three … " - Scrape loading dye transfer assay

Scrape loading dye transfer assay

Connective tissue growth factor promotes cell‐to‐cell communication …

WebSep 17, 2024 · The scrape loading/dye transfer assay was employed to access lucifer Yellow molecules transfer efficiency mediated by cell-cell communication. Connexin43 (Cx43), Pannexin1 (Panx1), MAPK, and the PI3K/Akt signaling pathway proteins were examined via Western blotting. Immunofluorescence was applied to visualize the … WebDec 22, 2024 · The scrape-loading dye transfer assay adapted from (Babica et al. 2016) was used to assess gap-junction intercellular communication within an EB. A clean linear cut was made across an individual EB plated on a glass coverslip using a curved surgical scalpel blade. The EB was then loaded with lucifer yellow fluorescent dye added into the culture ...

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WebFeb 1, 1987 · It employs scrape-loading to introduce a low molecular weight (MW) fluorescent dye, Lucifer yellow CH (MW 457.2) into cells in culture and allows the … Webin scrape-loading dye transfer assay [ 13] Packaging 25, 100 mg in glass bottle Bottomless glass bottle. Contents are inside inserted fused cone. Features and Benefits This …

WebFeb 7, 2024 · e Images obtained from the scrape loading (SL)/dye transfer (DT) assay after application of lucifer yellow CH dilithium salt (LY, MW 457), which transfers via the functional gap junction channels ... WebAug 20, 2024 · The scrape loading-dye transfer (SL-DT) technique is a simple assay for the functional evaluation of GJIC in various in vitro cultured mammalian cells and …

WebFigure 7 Utility of the scrape loading/dye transfer assay to detect a toxin/toxicant s ability to modulate gap junctional intercellular communication. The last two images (6ngmr TPA) illustrate both the epifluorescent and phase images, respectively, of the same cells to show that the complete inhibition of GJIC was not associated with the death ...

WebJul 31, 2024 · A scrape loading/dye transfer assay was performed to assess the GJIC function. The expression of connexins was examined by real-time reverse transcription polymerase chain reaction (RT-PCR), western blot and immunofluorescent staining.

WebVoltage is an important parameter that regulates the open probability of both intercellular channels (gap junctions) and undocked hemichannels formed by members of the connexin gene family. All ... diy child gate for stairsWebMar 1, 1987 · The scrape loading/dye transfer assay is a commonly used approach to study intercellular coupling via functional gap junction channels (el-Fouly et al., 1987). Gap … craig masbruch bankruptcyWebJan 20, 2024 · The commonly used technique for in vitro assessment of GJIC is so-called scrape loading-dye transfer assay (SL-DT) using a membrane-impermeable and gap … craig maryWebSep 3, 2024 · MC3T3-E1 cell line were cultured in a 25 cm2flask containing 3-4 ml α-MEM with 10% fetal bovine serum at 37 °C in a humidified atmosphere of 5% CO2in the air. FGF7 treatment Both primary osteoblasts and MC3T3-E1 cell line were seeded onto six-well plates at 5 × 105cells per well (85%~95% confluence). diy child play equipmentWebA scrape loading/dye transfer assay was used to assess the effects of the chemicals on GJIC. In this assay, a scratch is made in a confluent cell layer in the presence of a Lucifer … craig maschalWebFeb 28, 2011 · An innovative adaptation of traditional, fluorescence microscopy-based scrape loading-dye transfer (SL-DT) assay is presented, which has been optimized to simultaneously evaluate GJIC, cell density and viability, which could be included into in vitro screening and assessment of pharmacologically and toxicologically relevant compounds. … diy child car seat travel strapWebIt employs scrape-loading to introduce a low molecular weight (MW) fluorescent dye, Lucifer yellow CH (MW 457.2) into cells in culture and allows the monitoring of its … diy childproof cabinet locks