Pbad202/d-topo
Splet10. avg. 2024 · 生物在线 > 产品库 > 试剂库 > 载体及构建 > 细菌表达载体 > pbad102/d-topo大肠杆菌载体 概览 我的位置 ： 生物在线 产品库 试剂库 载体及构建 细菌表达载体 SpletMap of pBAD202/D/lacZ Description Map of pBAD202/D/lacZ . pBAD202/D/lacZ (7,520 bp) is a control vector that contains the gene for -galactosidase. The lacZ gene was amplified …
Pbad202/d-topo
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SpletThe recombinant S1-2 protein reacted specifically with both rabbit anti-histidine polyclonal antibodies and rabbit anti-PEDV polyclonal antibodies. Conclusion: Therefore, the recombinant S1-2 protein may be a useful tool for the development of a diagnostic test for PEDV or for a vaccine against PEDV. Keywords:
SpletTOPO™ Expression Vectors enable you to insert the exact DNA sequence you require simply by performing PCR with appropriately designed primers. Your PCR product is … SpletTOPO Cloning Vectors. Commonly used TOPO sequences including blunt, directional, and TOPO TA cloning vectors. Download Plasmid Set. Try SnapGene for Free. Download …
http://www.biofeng.com/zaiti/dachang/pBAD102-D-TOPO.html SpletTo determine whether pBAD202/D-TOPO could be used in S. oneidensis MR-1 cells for expression of multi-heme c-type cytochromes, two c-type cytochrome-encoding genes were cloned into pBAD202/D-TOPO according manufacturer’s instruction. They were mtrA of S. oneidensis MR-1 and DVU3171 of Desulfovibrio vulgaris Hildenborough (Table 1). Since
Spletwith pBAD202/D-TOPO (Invitrogen ) according to the manufacturer’s protocol. Briefly, purified PCR products were mixed with 1 µL of salt solution (1.2 M NaCl 2, 60 mM MgCl 2), 4 µL of pBAD202/D-TOPO and 1mL of distilled water. The ligation mixture was gently mixed and incubated at room temperature for 15 min and then
Splet大肠杆菌表达载体,载体序列,抗性,标签,图谱,以及应用 其他大肠杆菌表达载体： pBV221 pDsRed-Express2 pCold TF pCold I pBAD202/D-TOPO pBad/Myc-His B ptdTomato pBV220 pCold IV pE-SUMO pACYC184 pET-52b (+) pCold-GST pCold III pCold-ProS2 pBAD/Thio-TOPO pAmCyan pColdS-SUMO pCold II pBAD102/D-TOPO pBad/Myc-His C pBad/His B … nutrients in brown riceSpletWhat happens at gene expression when you add new associated to a gene governing network? Up answer this query, we profile 85 network rewirings included E. coli. Here we report that concerted patterns to differential expression dissemination from reconnected hub genes. The rewirings link promoter local to different transcription factor and σ-factor … nutrients in celery sticksSpletDirectional TOPO Cloning efficient cloning of blunt-ended PCR products generated with proofreading DNA polymerases. A choice of ampicillin (pBAD102/D-TOPO) or kanamycin … nutrients in celery seedSpletpBAD202/D-TOPO ® vector (Invitrogen, Carlsbad, CA, USA), and expressed in . Escherichia coli. The optimum concentration of arabinose and the optimum induction time for the expression of the recombinant S1-2 protein were determined using sodium dodecyl sulfate-polyacrylamide gel electrophoresis. The immunogenic reactivity of the recombinant S1 ... nutrients in cherry juiceSplet06. avg. 2024 · Amplified full-length PfMAG was ligated into expression vector pBAD202/D-TOPO (Invitrogen, USA). The constructed recombinant plasmid was verified by DNA sequencing and compared to its known P. falciparum 3D7 counterpart. nutrients in buckwheat groatsSplet30. maj 2024 · Instead of using the previously described ligase-based method, pBAD202/D-TOPO uses a highly efficient topoisomerase-based strategy for directional cloning of PCR … nutrients in chaga mushroomhttp://www.veterinaryworld.org/Vol.14/November-2024/10.pdf nutrients in chestnut mushrooms