NettetThe blocking reagent binds to endogenous mouse antibody in the tissue and prevents the binding of anti-mouse secondary antibody to those sites. - Incubate with primary antibody of your choice raised in mouse. Wash … Nettet6. Apply Super Block (ScyTek catalog# AAA), and incubate for 5 minutes at room temperature to block nonspecific background staining. Note: Do not exceed 10 minutes or there may be a reduction in desired stain. 7. Wash 1 time in buffer. 8. Apply Mouse-To-Mouse Block and incubate 10-60 minutes. Incubation time is dependent on the amount of
Vector Laboratories Vector M.O.M. Kit (Mouse-On-Mouse) Immunodetection ...
NettetM.O.M.™ Mouse Ig Blocking Reagent (MKB-2213) is used to block endogenous mouse antibody in the tissue section and is a key component of the M.O.M.™ Kits. Optimal staining results are obtained when this reagent is paired with the special M.O.M.™ Biotinylated Anti-Mouse Ig Reagent or the biotin-free, one-step micropolymer detection … NettetDescription. Mouse on Mouse (MOM) Blocking Reagent is a unique serum-free … golf flyer images
What is the composition of Vector M.O.M. Blocking Reagent?
NettetMouse IgG Blocking Reagent The M.O.M.™ Mouse Ig Blocking Reagent is a … Nettet16. aug. 2007 · The kit contains enough reagents for blocking 250 slides. Since the kit only provides the blocking reagents and the biotinylated antibody, the investigator is able to choose the detection method (e.g. peroxidase or fluorescent-based detection). I used the kit for staining mouse brain and spinal cord frozen sections. NettetKit De Mouse De Teclado Español Inalámbrico Bluetooth Portát. 472 pesos con 51 … golf flowers tyler the creator